Multiphoton Confocal Microscope Zeiss 710

Core Facilities

This system has been purchased with funds from Institute for Genomic Biology and a grant to Taekjip Ha from Howard Hughes Medical Institute. This system consists of a Zeiss 710 confocal scanner, Axio Observer Z1 microscope and a Spectraphysics Mai-Tai Ti-Sapphire laser.

The microscope has the following features:

Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm.
Deep See eHP laser, tunable from 690nm to1040nm with 70 fs pulses.
Twin gate beam splitter and spectral recycling loop for increased sensitivity.
QUASAR 34 channel spectral detector (2 standard PMTs and a 32 channel PMT array) with spectral resolutions up to 3 nm.
Two high sensitivity non de-scanned detectors (GaAsP-NDD).
Up to 6D acquisition (XYZTλP), XZ, YZ and Mosaics with simultaneous and sequential scan.
ZEN 2008 software for hardware control and spectral un-mixing; removal of auto-fluorescence.
Multi-Time/Physiology/FRET/FRAP software modules.
Up to 6000x6000 pixels per frame.
3D datasets can be rendered with Imaris and Analyze.