The Institute for Genomic Biology

Real-Time PCR

The Applied Biosystems 7900HT Fast Real-Time PCR System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. The interchangeable  384-well block capability combined with the optional Automation Accessory offers unmatched flexibility. Sample tracking is greatly simplified with the use of hand-held and integrated bar code readers. These features make the 7900HT system ideally suited to meet the high throughput requirements of today’s drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5’ nuclease assay. The 7900HT uses a Peltier-based thermal cycling system and an extended-life 488 nm argon-ion laser excitation source.

• Manufacturer: Applied Biosystems
• Equipment Model: #7900HT
• Location: 124 Institute for Genomic Biology
• Contact Person: Donna Epps, 244-9123
• *If you are interested in using this instrument, please see Tonya in Room 356 ERML for training.
• Fees & Consumables: see fees page
• Related Equipment: Eppendorf Centrifuge

Training Materials:

• Real-Time PCR Systems Chemistry Guide
• Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR