Structured illumination uses a spatially oscillating illumination to shift the spatial frequencies recorded by the camera to higher frequencies. This technique has twice the resolution of a confocal in X,Y, and Z. The Elyra system in the Core has 405nm, 488nm, 561nm, and 633nm lasers.
Image reconstruction can be run on Yellowstone in the Image analysis room.
links
- Virtual Tutorial
- References
- Principle Whitepaper
- Original Reference by M. G. L. GUSTAFSSON
- Sample Preparation Guide
Manufacturer: Zeiss
Equipment Model: Zeiss Elyra S1
Location: 121B, Carl R. Woese Institute for Genomic Biology
Contact Person: Austin Cyphersmith, 217-244-9654, ajcypher@igb.illinois.edu
Fees & Consumables: See fees page