Widefield Fluorescence Microscope
Widefield microscopy for fluorescence and transmitted light applications. Phase contrast, DIC, and FRET, and fluorescent filter options are available. An incubation chamber with CO2 with temperature controls is available for live cell imaging.
System Configuration
- Light source: Halogen lamp for bright-field microscopy, mercury lamp (X-Cite series 120 PC)
- Fluorescence Filter Cubes: DAPI, GFP, CY3, CY5
- FRET Filters: CFP, YFP
- Objectives: 10x/0.3 Dry, 20x/0.8 Dry, 40X/0.6 Dry, 40x/1.2 Water, 63x/1.4 Oil, 63 X/1.4 oil (Ph3)
- Cameras: Axiocam 506 color, Axiocam 506 b&w, and Orca Flash 4.0 v3 CMOS
- Acquisition software: Zen Black (Zen 2.3)
Manufacturer: Zeiss
Equipment Model: Zeiss Axiovert 200M
Location: 121C, Carl R. Woese Institute for Genomic Biology
Contact Person: Austin Cyphersmith, 217-244-9654, ajcypher@igb.illinois.edu
Fees & Consumables: See fees page
FRET Information: https://www.microscopyu.com/applications/fret/basics-of-fret-microscopy
Köhler Illumination: https://www-app.igb.illinois.edu/core-docs/books/igb-core-training/page/kohler-illumination
Training Materials:
- Axiovision Take Off Guide
- Instructions for Advanced Techniques
- Quick Reference Guide(pdf version)
