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Carl R. Woese Institute for Genomic Biology

Where Science Meets Society

Core Facilities

Zeiss LSM 880 AiryscanThe LSM 880 is a three-channel (1GAsP 2PMT) NLO ready spectral confocal system with Airyscan. This system is suitable for high resolution confocal imaging (170nm advertised, measured at 130nm at delivery) and is superior to the SR-SIM for samples with very large amounts of out-of-focus light such as pollen. For thin samples, the SR-SIM produces higher resolution images.

The microscopy has the following features:

  • Six visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 633nm.
  • Twin gate beam splitter and spectral recycling loop for increased sensitivity.
  • Up to 6D acquisition (XYZTλP), XZ, YZ and Mosaics with simultaneous and sequential scan.
  • ZEN 2012 software for hardware.
  • 3D datasets can be rendered with Imaris and Analyze.

Technical data on LSM 880

Tutorial on optical image formation, electronic signal processing and image digitization

Airyscan Quick reference guide

Zen-lite software download

Manufacturer: Zeiss

Equipment Model: Zeiss LSM 880

Location: 121A, Carl R. Woese Institute for Genomic Biology

Contact Person: Mayandi Sivaguru, 217-333-1214, sivaguru@igb.illinois.edu

Fees & Consumables: See fees page.

Sample Images:

           Confocal                                   Airyscan

Video: