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Carl R. Woese Institute for Genomic Biology

Where Science Meets Society

Core Facilities

Multiphoton Confocal Microscope Zeiss 710

This system has been purchased with funds from Institute for Genomic Biology and a grant to Taekjip Ha from Howard Hughes Medical Institute. This system consists of a Zeiss 710 confocal scanner, Axio Observer Z1 microscope and a Spectraphysics Mai-Tai Ti-Sapphire laser.

The microscope has the following features:

  • Seven visible excitation lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm.
  • Deep See eHP laser, tunable from 690nm to1040nm with 70 fs pulses.
  • Twin gate beam splitter and spectral recycling loop for increased sensitivity.
  • QUASAR 34 channel spectral detector (2 standard PMTs and a 32 channel PMT array) with spectral resolutions up to 3 nm.
  • Two high sensitivity non de-scanned detectors (GaAsP-NDD).
  • Fluorescence Life time Imaging (FLIM) from ISS
  • Up to 6D acquisition (XYZTλP), XZ, YZ and Mosaics with simultaneous and sequential scan.
  • ZEN 2008 software for hardware control and spectral un-mixing; removal of auto-fluorescence.
  • Multi-Time/Physiology/FRET/FRAP software modules.
  • Up to 6000x6000 pixels per frame.
  • 3D datasets can be rendered with Imaris and Analyze.

 

Technical data on LSM710

Tutorial on optical image formation, electronic signal processing and image digitization

Confocal image acquisition-Quick reference guide

Zen-lite software download

 

Manufacturer: Zeiss

Equipment Model: Zeiss LSM 710 NLO

Location: 121E, Carl R. Woese Institute for Genomic Biology

Contact Person: Mayandi Sivaguru, 217-333-1214, sivaguru@illinois.edu

Fees & Consumables: See fees page

Sample images: