Skip to main content

Illinois IGB

Core Facilities

Lightsheet

LIGHTSHEET MICROSCOPE-ZEISS

The Lightsheet Z1 system is a live cell and whole organ/scaffold imaging microscope. Compared to conventional fluorescence and confocal microscopy, this is the first system where the illumination axis and the detection axis are separated and placed orthogonal to each other. This system has two SCMOS cameras for simultaneous imaging in two channels and has the following specific features and advantages over fluorescence and confocal microscopes.

  • Orthogonal Plane Illumination of Excitation and Emission
  • Reduced Photobleaching and Phototoxicity
  • No Out of Focus Excitation
  • Live Cell Culture and Imaging with Temp and CO2 Controls
  • Large Whole Tissue or Animal Imaging
  • Lasers: 405, 488, 561 and 633 nm
  • Dual SCMOS Sensor over 2kx2k Resolution with Large Field of View 1.2x1.2 mm (20x)
  • Fast Acquisition for Time Lapse (~1000 slices full frame in ~3 min)
  • Single Side or Dual Side Illumination and Fusion of Multiple Angles
  • Ideal for Large Optically Cleared Specimens

Lightsheet Z1 Brochure

Lightsheet Sample Preparation Guide

System Principle and Pivot Scan References

Sample Images

Isosurface of Live and Dead Cells in a Scaffold of ~1 cubic mm

 Orthogonal Imaging of Plant Photosynthetic Components

  

Sample Video Click to play

 

Lightsheet Application and Zen Black Edition Lite Downloads

Contact Person: Kingsley Boateng, 217-300-1642, kboateng@illinois.edu

Fees & Consumables: See fees page.