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Illinois IGB

Core Facilities

Zeiss LSM 880 Airyscan

Zeiss LSM 880 Airyscan

Zeiss LSM 880 Confocal with Airyscan

The Zeiss LSM 880 is inverted confocal laser scanning microscope with Airyscan. This system is equipped with a unique scan head, incorporating a high-speed galvo scanner along with two PMTs and a 32 channel GaAsP-PMT area detector as well as a transmitted light PMT for DIC imaging. In addition, the Airyscan detector provides sub-diffraction limited imaging down to 140 nm (XY) and 400 nm (Z) resolution.

System Configuration:

  • Light source: Halogen lamp for bright-field microscopy, mercury lamp (X-Cite series 120 PC) for direct visualization of fluorescent specimens through the eyepiece
  • Fluorescence Filters cube for eyepiece viewing DAPY, FITC (green), DsRed, and Polarization
  • Laser Lines: Diode 405nm, Argon (458, 488, 514 nm), DPSS 561 nm, HeNe 633nm
  • Objectives: 10x/0.3 Dry, 25x?08 Dry, 20x/0.8 Dry, 40x/1.2 oil, 63x/1.4 Oil
  • Detectors: 2PMTs for fluorescence )ch1, ch3), 1 GaAs (ch2), Airyscan detector (chA), 1 PMT for Differential Interference Contrast (DIC) (T-PMT)
  • Motorized X, Y stage and Z galvo focusing stage
  • Incubation chamber x that allows for regulation of temperature, CO2 for live cell imaging
  • Acquisition software: Zen Black (Zen 2.3)

Manufacturer: Zeiss
Equipment Model: Zeiss LSM 880
Location: 121A, Carl R. Woese Institute for Genomic Biology
Contact Person: Austin Cyphersmith, 217-244-9654, ajcypher@illinois.edu
Fees & Consumables: https://www.igb.illinois.edu/corefacilities/fees
Video tutorial from Zeiss: https://www.zeiss.com/microscopy/us/local/zen-knowledge-base/zen-knowledge-base-lsm-700-780-880.html

Technical data on LSM 880

Tutorial on optical image formation, electronic signal processing and image digitization

Airyscan Quick reference guide

Zen-lite software download

 

Sample Images:

           Confocal                                   Airyscan

Video: