Microorganisms possess natural product biosynthetic gene clusters (BGCs) that may harbor unique bioactivities for use in drug development and agricultural applications. However, many uncharacterized microbial BGCs remain inaccessible. Researchers at Illinois previously demonstrated a technique using transcription factor decoys to activate large, silent BGCs in bacteria to aid in natural product discovery.

Researchers used single-molecule imaging to compare the genome-editing tools CRISPR-Cas9 and TALEN. Their experiments revealed that TALEN is up to five times more efficient than CRISPR-Cas9 in parts of the genome, called heterochromatin, that are densely packed. Fragile X syndrome, sickle cell anemia, beta-thalassemia and other diseases are the result of genetic defects in the heterochromatin.

An $87 million grant from the U.S. Department of Defense matched by more than $187 million in non-federal cost-share will fund collaborative efforts by a team of private and public entities, including the University of Illinois, to advance sustainable and reliable bioindustrial manufacturing technologies.

A team led by Steven L. Miller Chair professor of chemical and biomolecular engineering Huimin Zhao (BSD leader/CABBI/MMG) was awarded a five-year $20 million grant from the National Science Foundation (NSF) for the NSF Artificial Intelligence (AI) Institute for Molecular Discovery, Synthetic Strategy and Manufacturing (Molecule Maker Lab Institute or MMLI).

Researchers have identified key ingredients for producing high-value chemical compounds in an environmentally friendly fashion: repurposed enzymes, curiosity, and a little bit of light.

Scientists engineering valuable microbes for renewable fuels and bioproducts have developed a fast, efficient way to identify the most promising varieties.

Researchers have developed a triad of innovative tools to engineer low-pH-tolerant yeast Issatchenkia orientalis for production of valuable bioproducts from renewable biomass.

A paper published in Metabolic Engineering outlines the study’s three-pronged approach and its importance to the field of sustainable chemical production.

University of Illinois researchers achieved the highest reported rates of inserting genes into human cells with the CRISPR-Cas9 gene-editing system, a necessary step for harnessing CRISPR for clinical gene-therapy applications.

By chemically tweaking the ends of the DNA to be inserted, the new technique is up to five times more efficient than current approaches. The researchers saw improvements at various genetic locations tested in a human kidney cell line, even seeing 65% insertion at one site where the previous high had been 15%.

Genomic research has unlocked the capability to edit the genomes of living cells; yet so far, the effects of such changes must be examined in isolation. In contrast, the complex traits that are of interest in both fundamental and applied research, such as those related to microbial biofuel production, involve many genes acting in concert. A newly developed system will now allow researchers to fine-tune the activity of multiple genes simultaneously.

A new proof-of-concept study details how an automated system driven by artificial intelligence can design, build, test and learn complex biochemical pathways to efficiently produce lycopene, a red pigment found in tomatoes and commonly used as a food coloring, opening the door to a wide range of biosynthetic applications, researchers report.