Plant and Animal Genome XIII, San Diego, CA. Abstract P558.
Comparative mapping information can be effectively utilized for the identification of candidate genes within chromosomal regions containing ETL. The successful use of such information relies upon the construction of a detailed genome map determining segments of conserved synteny and gene order, as well as evolutionary breakpoints. The recent availability of the complete human genome sequence and thousands of homologous porcine sequences provides a tremendous resource for the construction of such a map of the porcine genome. Using the INRA-Minnesota porcine Radiation Hybrid (IMpRH) panel, we have constructed a radiation hybrid map composed of 2,272 markers, including 206 ESTs and 2,066 porcine BAC-end sequences (BESs). The average spacing between comparative anchor loci is 1.15 Mb based on human genome sequence. This radiation hybrid map has the highest resolution of any porcine genome map to date, and should greatly facilitate the positional cloning of porcine genes influencing traits of economic importance. Additionally, this map will provide a framework for anchoring contigs generated through BAC fingerprinting efforts as well as assist in the selection of a BAC minimal tiling path and assembly of the first sequence-ready map of the porcine genome.