Comparative Genomics, Illinois

University of Illinois at Urbana-Champaign

Abstracts

An Integrated BAC Physical Map Of The Pig Genome And Selection Of The Minimum Tilepath

Sean Humphray, Carol Scott, Richard Clark, Brandy Marron, Robert Plumb, Margarita Rogatcheva, Denis Milan, Patrick Chardon, Gary Rohrer, Dan Nonneman, Pieter de Jong, Stacy Meyers, Clare Bender, Alan Archibald, Jonathon Beever, Lawrence Schook, Jane Rogers

Plant and Animal Genomes Conference 2006, San Diego, CA. Abstract W237.

Abstract

We have constructed a physical map of the pig genome by integrating restriction fingerprints and BAC end sequences generated from 4 BAC libraries with radiation hybrid markers, and contig alignments to the human genome. The map provides coverage across the 18 pig autosomes and the X chromosome in 176 contigs as well as localised representation of the gene rich regions on Y. The map is accessible through WebFPC (http://www.sanger.ac.uk/Projects/S_scrofa/WebFPC/porcine/large.shtml) and represents an entry point for rapid electronic positional cloning of genes and fine mapping of QTLs. The map provides a platform for the selection of an efficient minimum tilepath through the genome to support clone-based sequencing and targeted functional genomics studies.