This image shows a coronal section of a whole rat larynx. Muscle fibers are outlined in green and type IIb muscle fibers are stained red in the paired lateral thyroaryntenoid muscles (part of the vocal folds).
Stains: Muscle fibers: BF-F3 antigen, diluted at 1:3 to label MHC IIb (Developmental Studies Hybridoma Bank, Iowa City, IA) with secondary Alexa594, goat/anti-rabbit IgG2b, diluted at 1:200 (Invitrogen: Molecular Probes, Eugene, OR). Muscle fiber outline: anti-laminin, diluted at 1:200 to outline muscle fibers (Sigma-Aldrich, St. Louis, MO) with secondary Alexa488, goat/anti-mouse IgG1, diluted at 1:200 (Invitrogen: Molecular Probes, Eugene, OR).
