Confocal image of an 8 micron serial section containing the developing leaf primordia in the grass Setaria viridis (ME34) at 8 days after germination. HCR-FISH labeling was used consisting of an mRNA positive control (red) in addition to DAPI which stains both the DNA contained in nuclei as well as the plant cell walls (blue).
Embedded with the Leica EG1150 H Embedding Station
Sectioned with Leica RM2255 rotary microtome
Image taken with LSM 710
Image courtesy of John Hodge and Min Ya of the Leakey and Min labs respectively.