Primary Human hepatic stellate cells cultured in synthetic polyethylene glycol gels with RGD for 5 days. Red: phalloidin actin stain. Green: antibody collagen stain. Blue: DAPI nucleus stain. Inset shows calcein-stained hepatic stellate cell (green) encapsulated in a synthetic polyethylene glycol gel embedded with fluorescent beads (blue). A healthy cell’s cytoskeleton is depolymerized using Cytochalasin D. Change in bead position is captured and analyzed along with the gel’s stiffness to calculate the three-dimensional traction force of the cell using Imaris software. System Zeiss LSM 880 with Imaris Software.
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Research
- Anticancer Discovery from Pets to People
- Biosystems Design
- Center for Advanced Bioenergy & Bioproducts Innovation
- Center for Genomic Diagnostics
- Center for Indigenous Science
- Center for Artificial Intelligence and Modeling
- Environmental Impact on Reproductive Health
- Genomic Ecology of Global Change
- Gene Networks in Neural & Developmental Plasticity
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- Infection Genomics for One Health
- Microbiome Metabolic Engineering
- Mining Microbiol Genomes
- Multi-Cellular Engineered Living Systems
- Regenerative Biology & Tissue Engineering