Carl R. Woese Institute for Genomic Biology

The IGB Core and Abberior will be hosting a hands on MINFLUX data analysis workshop November 6th. This remote workshop is exclusively for researchers who use the Illinois MINFLUX instrument. Attendees will perform MINFLUX imaging data analysis tasks on their own computers through the workshop.

Register here 

Description
Interactive workshop on MINFLUX data analysis with focus on imaging data. Participants will follow Jupyter notebooks for guided live data analysis. Follow-up workshop with focus on tracking data in planning.
 

Requirements
-    Basic understanding of MINFLUX and basic programming knowledge
-    Computer with functional Jupyter Lab
 

Provided
-    Jupyter notebook
-    Tutorial for installing Python and Jupiter Lab
 

Outline
8.00 am
WELCOME and MINFLUX DATA BASICS
Exported Data Fields
ZARR files
MINFLUX Metadata
 

8.30 am
BASIC MINFLUX POST PROCESSING
MINFLUX Data Quality
Z-Factor
Localization Information and Filtering
Trace Information and Filtering
Localization Precision
 

10.00 am
OPEN DISCUSSION ON FILTERING
 

10.30 am 
MULTICOLOR MINFLUX DATA
Exchange DNA PAINT
Spectral Classification
 

11.30 pm 
OPEN DISCUSSION ON DYES
 

12.00 pm 
Break
 

12.30 pm 
EXTRACTING SPATIAL INFORMATION
Clustering algorithms
Alphashape
Nearest Neighbor Distances
 

1.30 pm 
MINFLUX DATA ANALYSIS IN LITERATURE
 

2.20 pm 
OPENSOURCE TOOLS
 

2.30 pm
END

The 2026 Midwest Advanced MINFLUX and Super Resolution Workshop will be the week of July 20, 2026. Technology and research presentations will be Monday through Wednesday morning, with hands-on sessions in the evenings and all-day Thursday and Friday. In addition to our workshop, the 7th Midwest Single Molecule Workshop will be taking place on Wednesday afternoon, Thursday and Friday July 22nd to 24th at the Beckman Institute.