Carl R. Woese Institute for Genomic Biology

The IGB Core and Abberior will be hosting a hands-on MINFLUX tracking data analysis workshop March 5th. This remote workshop is exclusively for researchers who use the Illinois MINFLUX instrument. Attendees will perform MINFLUX tracking data analysis tasks on their own computers through the workshop. 

Register Here

Description
Interactive workshop on MINFLUX data analysis with focus on tracking data. Participants will follow Jupyter notebooks for guided live data analysis. Follow-up workshop with focus on tracking data in planning.

Requirements

• Basic understanding of MINFLUX and basic programming knowledge
• Computer with functional Jupyter Lab

Provided

• Jupyter notebook
• Tutorial for installing Python and Jupiter Lab on Windows

Outline

8.00 am 
WELCOME and MINFLUX DATA BASICS 

• Exported Data
• Fields ZARR files
• MINFLUX Metadata

8.30 am 
FUNDAMENTALS OF MINFLUX TRACKING MINFLUX 

• Tracking Technicalities
• MINFLUX Tracking Spatial-Temporal precision
• Hands On / Discussion

10.00 am 
TRACKING FILTERING 

• Basic trace metrics for tracking filtering
• DCR filtering
• Track Cutter
• Hands On / Discussion

12.00 pm 
Break

12.30 pm 
STEP FITTING 

• Step-finder algorithms
• Hands On/ Discussion

13.30 pm 
DIFFUSION COEFFICIENT 

• How to address MINFLUX heterogeneous sampling times
• Hands On / Discussion

2.30 pm 
MINFLUX TRACKING DATA ANALYSIS IN LITERATURE

3.00 pm 
END 
 

The 2026 Midwest Advanced MINFLUX and Super Resolution Workshop will be the week of July 20, 2026. Technology and research presentations will be Monday through Wednesday morning, with hands-on sessions in the evenings and all-day Thursday and Friday. In addition to our workshop, the 7th Midwest Single Molecule Workshop will be taking place on Wednesday afternoon, Thursday and Friday July 22nd to 24th at the Beckman Institute.