Minimal photon fluxes (MINFLUX) is a new single molecule localization microscopy (SMLM) technology that can provide resolutions in the range of 1 to 3 nm for structures in fixed and living cells by localizing individual switchable fluorophores with a probing donut-shaped excitation beam. The IGB Core hosted a Minflux Workshop on October 13, 2022 to introduce Midwest Researchers to the Minflux technology. Twenty seven people registered from eight institutions, with participants from the Midwest, Japan and Canada. We started with a description of the instrumentation, followed by talks on applications and sample preparation.
Here is the link to the video recording of the workshop: https://youtu.be/oAccDU4FuhE
Gopika Gopan, a PhD student from Martin Gruebele’s Lab discussing how their lab is using the technology to enhance their research.
Dr. Kingsley Boateng providing a live demo.
The Workshop concluded with a live demonstration of the tracking and imaging capabilities of the instrument, as well as the ability of outside users to ship samples and remotely image them.
You may visit our website for additional information on Minflux.