Synthesis of TALENs
Fully automated synthesis of TALEN libraries. (Chao et al., 2017)
(a) General workflow for the DNA assembly pipeline based on the Golden Gate method. Script Generator converted project design ideas, such as permutations of DNA parts, to assembly designs with appropriate extensions and further robotic commands for pipetting the stock plasmids to DNA mixes. In Golden Gate reactions, Type IIs restriction enzymes such as BsaI generated a set of standard precharacterized 4-bp single strained ends as linkers. The corresponding linkers annealed and were ligated by T4 ligase. (b) Process flow diagram for the build step. Unit operations employed were marked in blue.
High-throughput yeast strain engineering
Automated yeast strain engineering using iBioFAB (Si et al., 2017)
(a) Hardware layout of iBioFAB. (b) Process modules and unit operations in the workflow of yeast engineering. Any subset of all the unit operations enabled by iBioFAB can be programmed in custom-designed sequences to perform necessary process modules for creating and screening strain libraries in an iterative and automated manner. (c) Process flow diagram. Unit operations used in the yeast engineering workflow are marked in blue in b,c.