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Illinois IGB

Core Facilities

March 2018

A microfluidic gradient chamber (MGC) was used to study the effects of different exposure histories on the development of resistance in bacteria in a porous network. An array of micro-scale hexagon wells interconnected with channels was built into the MGC to emulate a porous network. A linear concentration gradient of antibiotic ciprofloxacin was created across the well array, where E. coli cells with GFP were inoculate. Response of E. coli cells in the ciprofloxacin concentration gradient was observed in real-time.