The image illustrates the collagen microstructure of a pig's digital flexor tendon, captured using second harmonic generation (SHG) microscopy in the longitudinal orientation. The image reveals the intricate crimping, or waviness, of the collagen fibers that contributes to the tendon's nonlinear mechanical behavior and facilitates its biological function. The scale bar is 20µm. The forward scattered SHG signal is shown in red and the back scattered SHG signal is shown in green. Imaged on the LSM 710 by Amir Ostadi Moghaddam in the Wagoner Johnson Lab.