The goal of this project is to understand the solute transport at the extracellular space of a rodent brain by intracranial injection. The tracer used is Texas red dextran 3 and it shows the convection flow path in the brain. The other label is the expression of the circadian gene (PER1) that has a VENUS reporter. This project is being funded by 1R61HL159948-01. The microscope used is the Miltenyi Ultramicroscope and image segmented with the IMARIS software.