Live Glioblastoma cells expressing a Mitochondrial ROS sensor from Rex Gaskins lab were imaged using the LSM 880 with Airyscan.  Imaris was used to create a nuclear surface and identify center of mass points for the mitochondria.  A MatLab Xtension was used to classify the mitochondrial center of masses by distance from the nucleus center of mass and color code them accordingly.  Colors are as follows: yellow = 0-20um, orange = 20-40um, blue = 40-60um, purple =80-100um, and pink >100um. MatLab Xtenstions greatly expand the functionality of Imaris, please contact Austin Cyphersmith (ajcypher@illinois.edu) if you have questions about using MatLab Xtensions for Imaris in your research.