"Representative coronal image from one hemisphere of an E16.5 mouse embryo brain section (25 μm thick) stained for NeuN (red) and Hoechst (blue). NeuN is a neuronal nuclear protein that serves as a marker of post-mitotic neurons, allowing identification of maturing neuronal populations. Hoechst is a fluorescent DNA-binding dye that labels all cell nuclei, providing structural context. Image was acquired using a Zeiss LSM 880 confocal microscope with a 20× objective. We thank Fernando Rigal for his assistance with microscope settings"
Image provided by Rafael J. Gonzalez-Ricon from the Antonson Lab